The AN703 antibody recognizes Dictyostelium discoideum PDI by immunofluorescence

Dictyostelium discoideum

Dictyostelium discoideum is a cellular slime mold [4] that serves as an important model organism [5] in a variety of fields. Cellular slime molds have an unusual life cycle. They exist as separate amoebae, but after consuming all the bacteria in their area they proceed to stream together to form a multicellular organism. These features make them a valuable tool for studying developmental proces...

Vacuolar H(+)-ATPase of Dictyostelium discoideum. A monoclonal antibody study.

A Dictyostelium membrane fraction rich in vacuolar proton pumps, previously described by Nolta et al. (J. Biol. Chem. 266, 18,318-18,323, 1991), was used as the immunogen for production of monoclonal antibodies. We obtained antibodies that recognized polypeptides of 100 kDa and 68 kDa, corresponding to the two largest subunits of the vacuolar proton pump. In indirect immunofluorescence experime...

Spontaneous turning behaviour by Dictyostelium discoideum slugs.

Dictyostelium discoideum slugs migrating in the absence of external stimuli tend to continue migrating in the original randomly 'chosen' direction. Spontaneous deviations from the starting direction accumulate with time at a rate that measures the 'physiological noise' in the slugs' steering mechanism. Most of the spontaneous turning signals arise in the transduction chain 'downstream' of the c...

Thermotaxis by pseudoplasmodia of Dictyostelium discoideum.

The temperature dependence of migration rate and of the thermotactic sensitivity of pseudoplasmodia of Dictyostelium discoideum has been measured. Migration rate increases with temperature to 20 degrees, is temperature insensitive from 20 degrees to 27.5 degrees, and decreases with temperature to 29 degrees, above which point migration ceases. However, pseudoplasmodia formed from cells grown at...

influx in Dictyostelium discoideum